Activation of dendritic cells by local ablation of hepatocellular carcinoma
- 作者：Mona Y. Ali ; Christian F. Grimm ; Marcus Ritter ; Leonhard Mohr ; Hans-Peter Allgaier ; Robert Weth ; Wulf O. Bocher ; Katja Endrulat ; Hubert E. Blum and Michael Geissler
- 刊名：Journal of Hepatology
- 出版年：2005
- 期刊代码：162_01688278
- 类别：med
- 出版时间：2005
- 卷：43
- 期：5
- 页码：817-822
- 文件大小：177 K
摘要
Background/Aims
Local ablation methods are an effective treatment for hepatocellular carcinoma (HCC). The rate of recurrence or development of intra-hepatic metastases may be lowered by antitumoral immune responses. Since HCCs are in general only weakly immunogenic, cell injury induced by local tumor ablation (PEI/RFTA) may increase HCC immunogenicity and may release endogenous adjuvants that activate dendritic cells (DC). The aim of the study, therefore, was the analysis whether PEI or RFTA induced injury results in an adjuvant effect for immune responses to HCCs.Methods
Eight HCC patients were treated with PEI or RFTA and serially analyzed for 4 weeks. Plasmocytoid (PDC) and myeloid dendritic cells (MDC) were analyzed directly ex vivo and in vitro using FACS and proliferation assays.
Results
HCC ablation induced a functional transient activation of MDC but not of PDC associated with increased serum levels of TNF-α and IL-1β.
Conclusions
These findings suggest that the combination of PEI or RFTA and active antigen specific immunotherapeutic approaches using DCs is a promising approach for the induction of sustained antitumoral immune responses aiming at the reduction of tumor recurrence and metastases in HCC patients.