Identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry

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• 作者：Hongyang Zhang^a ; Huihua Yang^b ; ^{yang98@guet.edu.cn} ; Min Zhang^c ; Yuerong Wang^a ; Jingrong Wang^d ; Leefong Yau^d ; Zhihong Jiang^d ; Ping Hu^a ; ^{huping@ecust.edu.cn}
• 关键词：Luo-Han-Guo ; Siraitia grosvenorii ; Food analysis ; Food composition ; Flavonol and triterpene glycosides ; Ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry ; Liquid chromatography&ndash ; mass spectrometry ; LC&ndash ; MS ; G
• 刊名：Journal of Food Composition and Analysis
• 出版年：2012
• 期刊代码：119_08891575
• 类别：abs
• 出版时间：March, 2012
• 卷：25
• 期：2
• 页码：142-148
• 文件大小：612 K

摘要

In this study, a method for the identification of flavonol and triterpene glycosides in Luo-Han-Guo extract using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) with personal database matching has been developed. Two flavonol glycosides and 20 triterpene glycosides were identified, and in addition the profiles of Luo-Han-Guo samples collected at different growing periods (15-, 40-, and 80-days) were compared. Principle component analysis (PCA) showed a clear separation among the various samples from different growing periods. The content of mogroside V significantly increased, while the level of mogroside IIE class dramatically decreased with an increase of growing time; however, the content of mogroside IV class was found to be high after a 40-day period of accumulation, but it subsequently decreased in the fully ripe period. Finally, 7 mogrosides could be screened as potential markers for discrimination of Luo-Han-Guo samples at different stages. The proposed analytical method combined with multivariate statistical analysis was shown to be a useful tool for investigating the chemical components of Luo-Han-Guo as well as for its quality evaluation.