Protective effect of extract of Acanthopanax senticosus harms on dopaminergic neurons in Parkinson's disease mice
- 作者：Shu-min Liu ; Xu-zhao Li hoogel@163.com ; Yan Huo ; Fang Lu ; lufang_19790501@163.com
- 关键词：MPTP-HCl ; 1-Methyl-4-phenyl-1 ; 2 ; 3 ; 6-tetrahydropyridine Hydrochloride ; DA ; Dopamine ; HVA ; Homovanillic acid ; UPLC&ndash ; ToF-MS ; Ultra-performance liquid chromatography combined with time-of-flight mass spectrometry ; BV/h ; (bed volume)/hour ; IOD ; Integral o
- 刊名：Phytomedicine
- 出版年：2012
- 期刊代码：259_09447113
- 类别：med
- 出版时间：15 May, 2012
- 卷：19
- 期：7
- 页码：631-638
- 文件大小：1423 K
摘要
To study the neuroprotective effect of extract of Acanthopanax senticosus Harms against MPTP-induced mice model of Parkinson's disease and its mechanism. The Parkinson's disease mice model was induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Hydrochloride (MPTP-HCl, 30 mg/kg daily for 5 days). High dose group and low dose group were medicated with extract of Acanthopanax senticosus Harms for 20 days, dose amounted to 182 mg/kg and 45.5 mg/kg daily respectively. The behavioral testing of mice was assessed using pole-climbing test. The levels of Dopamine (DA) and Homovanillic acid (HVA) in striatum were determined by Ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-ToF-MS). The levels of dopamine receptor 1 and 2 in striatum were assayed simultaneously with the help of immunohistochemical method. The level of Caspase-3 protein in substantia nigra was analyzed by Western Blot. From Day 5 during the administration of extract of Acanthopanax senticosus Harms, pole-climbing time in low and high dose group were significantly less than model group (p < 0.05). Compared with model group, the DA level of striatum in low dose group was significantly higher (p < 0.01), the number of dopamine receptor 1 and dopamine receptor 2-positive cells in low and high dose group were significantly less (p < 0.05), the Caspase-3 protein level of substantia nigra in low and high dose group were significantly less (p < 0.05). The neuroprotective effect of extract of Acanthopanax senticosus Harms may be able to protect C57BL/6 mice against MPTP-induced dopaminergic neuronal damage.