Isolation and structural characterization of a neutral polysaccharide from the stems of Dendrobium densiflorum

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• 作者：Qiang Li^a ; Yang Xie^a ; ^{yxie201203@126.com} ; Jianwei Su^a ; Qingsheng Ye^b ; Zhimin Jia^a
• 关键词：COSY ; correlation spectroscopy ; DDP ; Dendrobium densiflorum polysaccharide ; FT-IR ; Fourier transform infrared spectra ; GC&ndash ; MS ; gas chromatograph&ndash ; mass spectrometer ; HMBC ; heteronuclear multiple bond correlation ; HSQC ; heteronuclear spin quantum
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：1 June, 2012
• 卷：50
• 期：5
• 页码：1207-1211
• 文件大小：392 K

摘要

A novel neutral heteropolysaccharide (DDP-1-D) was purified from hot water extracts of dried stem of Dendrobium densiflorum by DEAE-52 and Sephacryl S-200 High-Resolution Chromatography. The heteropolysaccharide had an average molecular weight about 9440 Da. It was composed mainly of glucose and mannose in the ratio of 3.01:1. Structural features of DDP-1-D were elucidated by a combination of chemical and instrumental techniques, including FT-IR, GC-MS, periodate oxidation-Smith degradation, ¹H and ¹³C NMR spectroscopies (including COSY, TOCSY, HSQC, and HMBC spectra). The results indicated that DDP-1-D is a mannoglucan and has a backbone consisting of (1鈫?)-linked 伪-d-Glcp, (1鈫?)-linked 伪-d-Glcp, (1鈫?)-linked 伪-d-Manp and (1鈫?)-linked 尾-Manp. This is the first study to provide clear evidence for the structure of the polysaccharide in D. densiflorum.