Cardamonin exerts potent activity against multiple myeloma through blockade of NF-魏B pathway in vitro

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• 作者：You Qin^a ; ¹ ; Chun-Yan Sun^a ; ¹ ; Fu-Rong Lu^b ; Xiang-Rong Shu^a ; Di Yang^a ; Lei Chen^a ; Xiao-Mei She^a ; Nicholas Miller Gregg^c ; Tao Guo^a ; Yu Hu^a ; ^{dr_huyu@126.com}
• 关键词：AO ; acridine orange ; Bcl-2 ; B-cell lymphoma-2 ; Bcl-xL ; B-cell lymphoma-xL ; CCK-8 ; Cell Counting Kit-8 assay kit ; EB ; ethidium bromide ; cIAP ; cellular inhibitor of apoptosis ; ECL ; enhanced chemiluminescenece ; I魏B伪 ; Ikappa-B alpha ; IKK ; I魏B kinase ; MM ; m
• 刊名：Leukemia Research
• 出版年：2012
• 期刊代码：186_01452126
• 类别：med
• 出版时间：April, 2012
• 卷：36
• 期：4
• 页码：514-520
• 文件大小：946 K

摘要

NF-魏B plays a major role in the pathology of multiple myeloma. Here, we intended to investigate the regulating effect of cardamonin on NF-魏B in myeloma cells. We found for the first time that cardamonin suppressed viability and induced apoptosis of myeloma cells. Cardamonin activated caspase-3 and PARP and suppressed the expression of various anti-apoptotic proteins. We discovered that NF-魏B was repressed by cardamonin through suppression of IKK expression and I魏B伪 phosphorylation. Furthermore, the expression of NF-魏B-regulated gene products ICAM-1, COX-2 and VEGF was down-regulated by cardamonin. These results suggest that cardamonin blocks NF-魏B pathway in human multiple myeloma cells.