De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response
- 作者：Yan-Yuan Bao ; Ying Wang ; Wen-Juan Wu ; Dong Zhao ; Jian Xue ; Bao-Qin Zhang ; Zhi-Cheng Shen ; Chuan-Xi Zhang ; chxzhang@zju.edu.cn
- 关键词：Nilaparvata lugens ; Intestine transcriptome ; Illumina sequencing ; Digestion ; Immunity ; Detoxification
- 刊名：Genomics
- 出版年：2012
- 期刊代码：170_08887543
- 类别：bio
- 出版时间：April, 2012
- 卷：99
- 期：4
- 页码：256-264
- 文件大小：1279 K
摘要
The brown planthopper (Nilaparvata lugens, BPH) is the most serious rice plant pests in Asia. In this study, we performed transcriptome-wide analysis on BPH intestine. We obtained more than 26 million sequencing reads that were then assembled into 53,553 unigenes with a mean size of 388 bp. Based on similarity search with the nucleotide sequences available at NCBI, BPH intestine-specific transcriptome analysis identified 21,405 sequences. Assembled sequences were annotated with gene description, gene ontology and clusters of orthologous group terms. The digestion-, defense- and xenobiotic metabolism-related genes were abundantly detected in the transcripts from BPH intestine. Many novel genes including 33 digestion-related genes, 25 immune responsive genes and 27 detoxification-related genes are first reported here. We investigated the gene expression patterns at the transcript levels in different tissues by quantitative real-time PCR analysis, which revealed that some genes had intestine-specific expression, implicating their potential significance for BPH management.