Anyone for an aperitif? Yes, but only a Braulio DOC with its certified proteome
- 作者：Elisa Fasoli ; Alfonsina D'Amato ; Attilio Citterio ; Pier Giorgio Righetti ; piergiorgio.righetti@polimi.it
- 关键词：CPLL ; combinatorial peptide ligand library ; PM ; ProteoMiner.
- 刊名：Journal of Proteomics
- 出版年：2012
- 期刊代码：P37_18743919
- 类别：ch
- 出版时间：18 June, 2012
- 卷：75
- 期：11
- 页码：3374-3379
- 文件大小：1085 K
摘要
The trace proteome of a Braulio aperitif (a 21%alcohol beverage, named after a mountain in the Val di Stelvio, Italy) has been investigated via capture with combinatorial peptide ligand libraries (CPLL, ProteoMiner). This aperitif is made with an infusion of 13 mountain herbs and berries, among which four are officially indicated in the label: Achillea moschata, juniper (Juniperus communis subsp. alpina) berries, absinthe (Artemisia absinthium) and gentian (Gentiana alpina) roots. Via capture with CPLLs at pH 7.0 and 2.2 we were able to identify 29 unique gene products, among which the PR5 (parasite resistance) allergen Jun r 3.2, a 25 kDa species from Juniperus rigida. Due to the paucity of data on these alpine herbs, it was difficult to attribute these proteins to the specific plant extracts presumably present in this beverage; however most of the species identified indeed belong to alpine herbs and plants, living in a habitat between 1000 and 2000 m of elevation. Most of them are enzymes, spanning a Mr range from 10 to 65 kDa. It is hoped that such a proteomic signature should help tracking counterfeited products sold on the market.