Cell culture-adapted IBDV uses endocytosis for entry in DF-1 chicken embryonic fibroblasts

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• 作者：Chi Wai Yip ; Chung Chau Hon ; Fanya Zeng ; Frederick Chi Ching Leung ; ^{fcleung@hkucc.hku.hk}
• 关键词：IBDV ; Calcium ; Clathrin-independent endocytosis ; Macropinocytosis
• 刊名：Virus Research
• 出版年：2012
• 期刊代码：92_01681702
• 类别：imm
• 出版时间：April, 2012
• 卷：165
• 期：1
• 页码：9-16
• 文件大小：712 K

摘要

Although membrane perforation was suggested as the means of penetration mediated by IBDV, the cellular mechanism being hijacked to facilitate its entry is largely unknown. In this study, the entry pathway of cell culture adapted IBDV (caIBDV) was characterized in DF-1 chicken embryonic fibroblasts. We observed that the entry of caIBDV was inhibited by bafilomycin A1 and CaEGTA which interfere with the function of vacuolar H⁺-ATPase (V-ATPase) and retain endosomal Ca²⁺. This result suggests that the intact caIBDV particle was transported to the V-ATPase positive vesicles for uncoating and implicates an essential role of endocytosis during the viral entry. The IBDV-mediated endocytosis was demonstrated to be clathrin-independent. Instead, the entry of caIBDV in DF-1 was reduced under the inhibitions or depletions of lipid raft, c-Src tyrosine kinase, dynamin and actin polymerization. In summary, this study confirmed the role of endocytosis in caIBDV entry and characterized the route of its endocytosis.