pH dependent growth of poly(L-lysine)/poly(L-glutamic) acid multilayer films and their cell adhesion properties
- 作者：Richert ; Ludovic ; Arntz ; Youri ; Schaaf ; Pierre ; Voegel ; Jean-Claude ; Picart ; Catherine
- 关键词：Self-assembly ; Biological molecules 脙脙脙脙脙脙 proteins ; Adhesion ; Atomic force microscopy ; Surface structure ; morphology ; roughness ; and topography
- 刊名：Surface Science
- 出版年：2004
- 期刊代码：104_00396028
- 类别：ch
- 出版时间：October 10, 2004
- 卷：570
- 期：1-2
- 页码：13-29
- 文件大小：533 K
摘要
The short-term interaction of chondrosarcoma cells with (PGA/PLL) polyelectrolyte multilayers was investigated in a serum-containing medium for films built at different pHs and subsequently exposed to the culture medium. The buildup of the films and their stability was first investigated by means of optical waveguide lightmode spectroscopy, quartz crystal microbalance, streaming potential measurements and atomic force microscopy. While film growth is linear at all pHs, after a few layers have been deposited the growth is much larger for the films built at basic pH and even more pronounced for those built at acidic pH. However, these latter films remain stable in the culture medium only if they have been crosslinked prior to the ionic strength and pH jumps. The films built at acidic pH were found to swell in water by about 200%whereas those built at other pHs did not swell in a physiological buffer. For thin films (芒芒芒20 nm) built at pH=7.4, the detachment forces were dependent on the outermost layer, the forces being significantly higher on PLL-ending films than on PGA-ending ones. In contrast, for the thick films built at pH=4.4 and at pH=10.4 (thickness of the order of few hundred of nanometers), the detachment forces were independent of the outermost layer of the film. The films built at pH=10.4, which shrink in contact with salt containing solutions, were highly cell adhesive whereas those built at acidic pH were highly cell resistant. Protein adsorption and film roughness (as measured by AFM) could not explain these striking differences. The high adhesion observed on the film built at pH 10.4 may rather be related to the secondary structure of the film and to its relatively low swellability in water, whereas the cell resistance of the films built at pH 4.4 may be linked to their high swellability. Therefore, for the PGA/PLL films, the cell adhesion properties can be tuned depending on the deposition pH of the polyelectrolyte solutions. This study reveals the importance of the multilayer structure and architecture to control the detachment force of cells onto such films.