Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo

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• 作者：Nahoko Shinohara^a ; Tsuyoshi Tsuduki^a ; ^{tsuzukit@biochem.tohoku.ac.jp} ; Junya Ito^a ; Taro Honma^a ; Ryo Kijima^a ; Soko Sugawara^a ; Tatsuya Arai^a ; Masao Yamasaki^b ; Aya Ikezaki^b ; Marino Yokoyama^b ; Kazuo Nishiyama^b ; Kiyotaka Nakagawa^c ; Teruo Miyazawa^c ; Ikuo Ikeda^a
• 关键词：BHT ; butylated hydroxytoluene ; BSA ; bovine serum albumin ; 伪-CDA ; 伪-calendic acid ; 尾-CDA ; 尾-calendic acid ; CLA ; conjugated linoleic acid ; CLnA ; conjugated linolenic acid ; CPA ; catalpic acid ; 伪-ESA ; 伪-eleostearic acid ; 尾-ESA ; 尾-eleostearic acid ; JA
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：2012
• 期刊代码：18_13881981
• 类别：bio
• 出版时间：July, 2012
• 卷：1821
• 期：7
• 页码：980-988
• 文件大小：1521 K

摘要

In this study, we compared the cytotoxic effects of natural conjugated linolenic acids (CLnAs) on human adenocarcinoma cells (DLD-1) in vitro, with the goal of finding CLnA isomers with strong cytotoxic effects. The antitumor effect of the CLnA with the strongest cytotoxic effect was then examined in mice. The results showed that all CLnA isomers have strong cytotoxic effects on DLD-1 cells, with jacaric acid (JA) having the strongest effect. Examination of the mechanism of cell death showed that CLnAs induce apoptosis in DLD-1 cells via lipid peroxidation. The intracellular levels of incorporated CLnAs were measured to examine the reason for differences in cytotoxic effects. These results showed that JA was taken into cells efficiently. Collectively, these results suggest that the cytotoxic effect of CLnAs is dependent on intracellular incorporation and induction of apoptosis via lipid peroxidation. JA also had a strong preventive antitumor effect in vivo in nude mice into which DLD-1 cells were transplanted. These results suggest that JA can be used as a dietary constituent for prevention of cancer.