Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene

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• 作者：Joã ; o Rodrigo Gil de los Santos^a ; ^{joao.gil@ufpel.edu.br} ; Otá ; vio Brod Storch^b ; Cristina Gevehr Fernandes^c ; Carlos Gil-Turnes^a ; ^b
• 关键词：Pichia pastoris ; Recombinant Pichia pastoris ; Probiotic ; Alpha toxin ; Clostridium perfringens ; Avian Necrotic Enteritis
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：4 May, 2012
• 卷：156
• 期：3-4
• 页码：448-451
• 文件大小：127 K

摘要

The probiotic properties of Pichia pastoris and of a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene were evaluated in broilers. One-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials. The control group (1) received plain food, while the other groups were supplemented with either P. pastoris (2), the recombinant P. pastoris (3) or Bacillus cereus var. Toyoi (4). At day 49, live weights, feed efficiency and seroconversions were higher (P < 0.05) in the supplemented groups than in the control groups. Group 3 showed the best results, while group 2 had lower weight gain than groups 3 and 4 although food conversion was better than in group 4. Seroconversions were not different (P > 0.05) among the supplemented groups. Adverse reactions were not observed in histopathologic evaluation. We concluded that P. pastoris and the recombinant P. pastoris could be used as probiotics in broilers.