Comparative high-performance liquid chromatography enantioseparations on polysaccharide based chiral stationary phases prepared by coating totally porous and core-shell silica particles

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• 作者：Ketevan Lomsadze^a ; George Jibuti^a ; Tivadar Farkas^b ; Bezhan Chankvetadze^a ; ^{bezhan_chankvetadze@yahoo.com}
• 关键词：Enantioseparations ; Chiral HPLC ; Core&ndash ; shell type chiral stationary phases ; Polysaccharide-based chiral stationary phases
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：20 April, 2012
• 卷：1234
• 期：Complete
• 页码：50-55
• 文件大小：776 K

摘要

This article reports comparative high-performance liquid chromatographic separations of enantiomers with chiral stationary phases (CSPs) prepared by coating cellulose tris(4-chloro-3-methylphenylcarbamate) on totally porous and on core-shell type silica of comparable particle diameter. Several interesting observations were made: (1) the selectivity of separation was higher on core-shell type CSP compared to totally porous CSP at comparable content of chiral selector (polysaccharide derivative); (2) much flatter dependence of plate height on the mobile phase flow rate was observed for columns packet with CSP prepared with core-shell silica compared to the ones packed with CSPs prepared with totally porous particles; (3) at low mobile phase flow rates core-shell CSP provided lower resolving ability compared to a commercially available CSP having four times higher content of chiral selector along with higher retention of chiral analytes. However, at high flow rates core-shell type CSP performed similarly or better than the commercial column in regards of plate count (N) and peak resolution (R_s) per column length and within a given total analysis time. The advantage of CSP prepared with core-shell silica is obvious from the viewpoint of plate numbers and resolution calculated per unit time (i.e. speed of analysis).