Tumor Promoter Benzoyl Peroxide Induces Sulfhydryl Oxidation in Protein Kinase C: Its Reversibility Is Related to the Cellular Resistance to Peroxide-Induced Cytotoxicity

详细信息	查看全文 | 推荐本文 |

• 作者：Gopalakrishna ; Rayudu ; Gundimeda ; Usha ; Anderson ; Wayne B. ; Colburn ; Nancy H. ; Slaga ; Thomas J.
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：1999
• 期刊代码：116_00039861
• 类别：ch
• 出版时间：March 15, 1999
• 卷：363
• 期：2
• 页码：246-258
• 文件大小：141 K

摘要

Since tumor promoter benzoyl peroxide (BPO) mimics phorbol esters in some aspects, its effects on protein kinase C (PKC) were previously studied. However, in those studies due to the presence of thiol agents in the PKC preparations, the sensitive reaction of BPO with redox-active cysteine residues in PKC was not observed. In this study, by excluding thiol agents present in the purified PKC preparation, low concentrations of BPO modified PKC, resulting in the loss of both kinase activity and phorbol ester binding (IC₅₀