Enhancing the value of nitrogen from rapeseed meal for microbial oil production

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• 作者：Esra Uç ; kun Kiran^a ; ^b ; ¹ ; ^{esrauckun@yahoo.com} ; Apilak Salakkam^a ; ^{apilak.salakkam@live.com} ; Antoine P. Trzcinski^a ; ^{antoinetrzcinski@hotmail.com} ; Ufuk Bakir^b ; ¹ ; ^{ufukb@metu.edu.tr} ; Colin Webb^a ; ^{colin.webb@manchester.ac.uk}
• 关键词：Rapeseed meal ; Fermentation ; Solid state fermentation ; Free amino nitrogen ; Rhodosporidium toruloides ; Microbial oil
• 刊名：Enzyme and Microbial Technology
• 出版年：2012
• 期刊代码：26_01410229
• 类别：ce
• 出版时间：10 May, 2012
• 卷：50
• 期：6-7
• 页码：337-342
• 文件大小：421 K

摘要

Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g^鈭? dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g^鈭?), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g^鈭?), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g^鈭?), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g^鈭?).

The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3 g dry cell L^鈭? of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%. The dry cell weight obtained was higher than that obtained using conventional yeast extract, due to a higher total nitrogen content in the novel biomedium. The fatty acids obtained from the microbial oil were similar to those derived from rapeseed oil.