Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies
- 作者：Krystyna Pietrucha
- 关键词：Collagen ; Hyaluronic acid ; Thermal stability
- 刊名：International Journal of Biological Macromolecules
- 出版年：2005
- 期刊代码：62_01418130
- 类别：abs
- 出版时间：2005
- 卷：36
- 期：5
- 页码：299-304
- 文件大小：236 K
摘要
This report describes the effect of temperature on the mechanical viscoelastic properties such as: storage modulus (E′), loss modulus (E″), and loss tangent (tan δ) of the collagen sponges modified with hyaluronic acid (HA). In order to detect collagen–HA copolymer denaturation and to assess its thermal stability, the differential scanning calorimetry (DSC) supplemented by thermogravimetric (TG) measurements was used. The denaturation temperature (Td) of unmodified collagen samples increased from 69 to 86 °C for cross-linked samples, respectively. These temperature dependencies show remarkable changes in E′ and E″ at selected temperature up to 226 °C for all samples due to the release of loosely and strongly bound water. The influence of HA on the viscoelastic behavior of collagen is manifested by a shift of the tan δ peak associated with the process of decomposition towards higher temperatures resulting in a higher thermo-stability of the modified scaffolds.