Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment
- 作者：Yu-Qing Jin ; Wei Liu ; Tan-Hui Hong ; Yilin Cao
- 关键词：Schwann cells ; Purification ; Differential detachment ; Collagenase ; Nerve regeneration
- 刊名：Journal of Neuroscience Methods
- 出版年：2008
- 期刊代码：33_01650270
- 类别：neu
- 出版时间：15 May 2008
- 卷：170
- 期：1
- 页码：140-148
- 文件大小：1647 K
摘要
Schwann cell purification is usually difficult due to the contamination of fibroblasts, which often become a predominant cell type in Schwann cell culture in vitro. We have developed a novel and efficient method to enrich Schwann cells by differential detachment of two types of cells. In the culture, cells were treated with a multiplex collagenase and the Schwann cells were found to detach faster than fibroblasts and thus Schwann cells could be easily isolated. Within 5 days, Schwann cell purity could reach above 99%, which was confirmed by immunostaining characterization and flow cytometric analysis. In addition, this efficient method can reach a high cell yield after two rounds of differential detachment procedures and does not require antimitotic treatment or special equipments as often needed by other reported methods.