Comparison of the fast gradient performance of new prototype silica monolithic columns and columns packed with fully porous and core-shell particles

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• 作者：Fabrice Gritti^a ; Nobuo Tanaka^b ; Georges Guiochon^a ; ^{guiochon@ion.chem.utk.edu} ; ^{guiochon@utk.edu}
• 关键词：Fast chromatography ; Monolithic columns ; Particulate columns ; Gradient elution ; Extra-column band broadening ; Peak capacity
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：4 May, 2012
• 卷：1236
• 期：Complete
• 页码：28-41
• 文件大小：1081 K

摘要

The gradient elution performance of narrow-bore 2.3 mm 脳 50 mm (N733) and wider bore 3.2 mm 脳 50 mm (N648 and N655) prototype silica monolithic columns was investigated and compared to the performance of commercially available columns packed with sub-2 渭m fully porous particles (2.1 mm 脳 50 mm, 1.7 渭m BEH-C₁₈, Waters) and sub-3 渭m superficially porous particles (2.1 mm 脳 50 mm, 2.7 渭m Halo-ES-Peptide-C₁₈ (AMT), 1.7 and 2.6 渭m Kinetex-C₁₈, Phenomenex). Results show that the two wide monolithic columns show peak capacities similar to the one measured for the Kinetex column. In contrast, the narrow-bore monolithic column delivers a lower performance (鈭?0%) than the BEH, the Halo and the Kinetex columns. This work stresses out the importance of reducing the extra-column band broadening contribution of HPLC instruments when short 2.1 mm I.D. columns are used. The part of the instrument contribution originating downstream the column is important for all compounds; the one originating upstream the column is significant only for weakly retained compounds.