Genotoxicity evaluation of fullerene C₆₀ nanoparticles in a comet assay using lung cells of intratracheally instilled rats

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• 作者：Makoto Ema^a ; ^{ema-makoto@aist.go.jp} ; Jin Tanaka^b ; Norihiro Kobayashi^a ; Masato Naya^a ; Shigehisa Endoh^c ; Junko Maru^c ; Masayo Hosoi^b ; Miho Nagai^b ; Madoka Nakajima^b ; Makoto Hayashi^b ; Junko Nakanishi^a
• 关键词：Nanomaterials ; Fullerenes ; Genotoxicity ; Comet assay ; Lung ; Intratracheal instillation ; Inflammation
• 刊名：Regulatory Toxicology and Pharmacology
• 出版年：2012
• 期刊代码：57_02732300
• 类别：pha
• 出版时间：April, 2012
• 卷：62
• 期：3
• 页码：419-424
• 文件大小：475 K

摘要

The genotoxicity of fullerene C₆₀ nanoparticles was evaluated in vivo with comet assays using the lung cells of rats given C₆₀ nanoparticles. The C₆₀ nanoparticles were intratracheally instilled as a single dose at 0.5 or 2.5 mg/kg or repeated dose at 0.1 or 0.5 mg/kg, once a week for 5 weeks, to male rats. The lungs were obtained 3 or 24 h after a single instillation and 3 h after repeated instillation. Inflammatory responses were observed in the lungs obtained 24 h after a single instillation at 2.5 mg/kg and repeated instillation at 0.5 mg/kg. Histopathological examinations revealed that C₆₀ nanoparticles caused slight changes including hemorrhages in alveoli and the cellular infiltration of macrophages and neutrophils in alveoli. In comet assays using rat lung cells, no increase in%Tail DNA was found in any group given C₆₀ nanoparticles. These findings indicate that C₆₀ nanoparticles had no potential for DNA damage in comet assays using the lungs cells of rats given C₆₀ even at doses causing inflammation.