A new chitosan-thymine conjugate: Synthesis, characterization and biological activity

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• 作者：Santosh Kumar^a ; Joonseok Koh^a ; ^{ccdjko@konkuk.ac.kr} ; Hyerim Kim^a ; M.K. Gupta^b ; P.K. Dutta^c
• 关键词：Chitosan ; Thymine-1-yl acetic acid ; Acylation ; Conjugate ; Biomedical applications
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：1 April, 2012
• 卷：50
• 期：3
• 页码：493-502
• 文件大小：1390 K

摘要

Conjugation of chitosan with nucleobases is expected to expand its not only antimicrobial activity but also anti-cancer activity. Here, we report the synthesis of a novel chitosan-thymine conjugate by the reaction between chitosan and thymine-1-yl-acetic acid followed by acylation. The synthesized conjugate was characterized by FTIR, XRD, ¹H NMR, TGA and SEM. The microbiological screening results demonstrated the antimicrobial activity of the conjugate against bacteria viz., Escherichia coli, Staphylococcus aureus, and fungi viz., Aspergillus niger. The chitosan-thymine conjugate also inhibited (p < 0.05) the proliferation of human liver cancer cells (HepG2) in a dose-dependent manner but had no cellular toxicity in non-cancerous mouse embryonal fibroblast cells (NIH 3T3). Thus, the chitosan-nucleobase conjugate may open a new perspective in biomedical applications.