Modulation of oxidative stress by 纬-glutamylcysteine (GGC) and conjugated linoleic acid (CLA) isomer mixture in human umbilical vein endothelial cells

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• 作者：Yukiko K. Nakamura^a ; Michael A. Dubick^b ; Stanley T. Omaye^a ; ^{omaye@cabnr.unr.edu} ; ^{omaye@unr.edu}
• 关键词：纬-Glutamylcysteine ; Glutathione ; Conjugated linoleic acid ; Oxidative stress
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：June, 2012
• 卷：50
• 期：6
• 页码：1854-1859
• 文件大小：420 K

摘要

Individually, 纬-glutamylcysteine (GGC), a dipeptide and precursor of glutathione (GSH), and conjugated linoleic acid (CLA), a trans-fatty acid, exhibit antioxidant properties. The objective of this study was to compare effects of co-administration of GGC and CLA to treatment with GGC alone on oxidative stress and GSH synthesis in human endothelial cells. Changes in levels of 8-epi-PGF2伪, thiobarbituric acid reactive substances (TBARS), GSH, total antioxidants, GSH synthetase (GSS) expression, and transcription factor DNA binding were assessed in human umbilical vein endothelial cells (HUVEC) treated with GGC alone (100 渭mol/L) or combined with CLA isomer mixture (10, 50, 100 渭mol/L) for 24 h. Significantly higher levels of TBARS, 8-epi-PGF2伪, GSH, and GSS protein were found in cells treated with GGC and 10 渭mol/L CLA, compared to cells treated with GGC alone, indicative of prooxidant effects of CLA. Approximately 40%cell death was microscopically observed in cells incubated with GGC and 100 渭mol/L CLA. Despite lower levels of GSH, treatment with GGC and 50 渭mol/L CLA appeared to be protective from oxidative stress similar to treatment with GGC alone, as indicated by lower levels of TBARS, compared to control cells not treated with GGC and CLA.