Semi-continuous detection of toxic hexavalent chromium using a sulfur-oxidizing bacteria biosensor

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• 作者：Anup Gurung^a ; Sang-Eun Oh^a ; ^{ohsangeun@kangwon.ac.kr} ; Ki Duck Kim^b ; Beom-Soo Shin^b ; ^{bshin@kangwon.ac.kr}
• 关键词：Biosensor ; Sulfur-oxidizing bacteria ; Recovery ; Toxicity ; Stream water
• 刊名：Journal of Environmental Management
• 出版年：2012
• 期刊代码：82_03014797
• 类别：env
• 出版时间：15 September, 2012
• 卷：106
• 期：Complete
• 页码：110-112
• 文件大小：451 K

摘要

Toxicity testing is becoming a useful tool for environmental risk assessment. A biosensor based on the metabolic properties of sulfur-oxidizing bacteria (SOB) has been applied for the detection of toxic chemicals in water. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid under aerobic conditions. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. Five hours after Cr⁶⁺ was added to the SOB biosensor operated in semi-continuous mode (1聽min rapid feeding and 29聽min batch reaction), a decrease in effluent EC and an increase in pH (from 2-3 to 6) were detected due to Cr⁶⁺ toxicity to SOB. The SOB biosensor is simple; it can detect toxic levels of Cr⁶⁺ on the order of minutes to hours, a useful time scale for early warning detection systems designed to protect the environment from further degradation.