Semi-continuous production of 2-keto-gluconic acid by Pseudomonas fluorescens AR4 from rice starch hydrolysate

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• 作者：Wen-Jing Sun^a ; ^b ; ¹ ; ^{sunwenjing1919@163.com} ; Yan-Zheng Zhou^a ; ^b ; Qiang Zhou^b ; Feng-Jie Cui^a ; ^b ; ¹ ; ^{fengjiecui@163.com} ; Shi-Lian Yu^b ; Lei Sun^c
• 关键词：2-Keto-gluconic acid (2KGA) ; Pseudomonas fluorescens ; Rice starch hydrolysate ; Semi-continuous fermentation
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：April, 2012
• 卷：110
• 期：Complete
• 页码：546-551
• 文件大小：261 K

摘要

2-Keto-gluconic acid (2KGA) was produced in a semi-continuous process using Pseudomonas fluorescens AR4 and rice starch hydrolysate (RSH). The bacterium was cultured in medium with an initial glucose concentration of 170 g/L supplied as RSH. Once the glucose level had dropped to 20 g/L, 60%of the culture volume was replaced with fresh medium containing 190 g/L of glucose in the form of RSH. After an additional two cycles of growth and media replacement, a total of 476.88 g/L of glucose was consumed and 444.96 g/L of 2KGA was produced. A total productivity of 6.74 g/L and a yield of 0.93 g/g were obtained. These findings suggest that P. fluorescens AR4 is suitable for the production of commercially acceptable levels of 2KGA in semi-continuous culture.