Structural characterization of 13²-hydroxy-(13²-S)-phaeophytin-a from leaves and stems of Amaranthus tricolor isolated by high-speed countercurrent chromatography

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• 作者：Gerold Jerz ; Tabiwang N. Arrey ; Victor Wray ; Qizhen Du ; Peter Winterhalter
• 关键词：Amaranthus tricolor ; Amaranthaceae ; 13²-hydroxy-(13²-S)-phaeophytin-a ; chlorophyll b methoxylactone ; Countercurrent chromatography ; 1D- and 2D-NMR spectroscopy ; EI-MS ; ESI-MS/MS
• 刊名：Innovative Food Science and Emerging Technologies
• 出版年：2007
• 期刊代码：57_14668564
• 类别：abs
• 出版时间：September 2007
• 卷：8
• 期：3
• 页码：413-418
• 文件大小：488 K

摘要

Amaranthus tricolor (Amaranthaceae) is a popular vegetable in China. Separation of lipophilic extracts of the aerial parts of A. tricolor by means of preparative ‘High-speed Countercurrent Chromatography’ (HSCCC) led to the isolation of two chlorophyll breakdown products which were characterized as 13²-hydroxy-(13²-S)-phaeophytin-a (1) and chlorophyll b methoxylactone (2).

The structure of 1 was established using a combination of 1D- and 2D-NMR spectroscopic and mass spectrometric techniques. Component 2 was identified by means of ESI-MS/MS analysis.

Industrial relevance

Chlorophylls and their degradation products are natural pigments of interesting bioactivities. For industry, recovery of these pigments from waste materials of fruit and vegetable processings could be a promising by-product line. Here we present the isolation of a pure chlorophyll breakdown product which is in principle suited as a nutraceutical ingredient. Large-scale isolation procedures using countercurrent chromatography techniques for chlorophyll related pigments are currently under development.