A novel conductometric creatinine biosensor based on solid-state contact ammonium sensitive PVC-NH₂ membrane

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• 作者：Ibrahim Isildak^a ; ^{iisildak@gmail.com} ; ^{isildak@yildiz.edu.tr} ; Osman Cubuk^b ; Melda Altikatoglu^c ; Murat Yolcu^b ; Vildan Erci^b ; Nihat Tinkilic^b
• 关键词：Conductometric biosensor chip ; Creatinine ; Biosensor ; Creatininase immobilization ; Solid-state biosensor
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 March, 2012
• 卷：62
• 期：Complete
• 页码：34-38
• 文件大小：455 K

摘要

A novel, highly sensitive and stable conductometric biosensor for creatinine determination is developed. The biosensor is based on solid-state contact ammonium-sensitive sensor. Creatininase is chemically immobilized on the surface of the solid-state contact ammonium-sensitive membrane via glutaraldehyde covalent attachment method. The developed conductometric creatinine biosensors demonstrate high sensitivity and short response time toward creatinine. The detection limit of the biosensor was about 2 脳 10^鈭? M and the response time was shorter than 10 s in phophate buffer solution at pH 7.20. The linear dynamic range of the biosensor was between 1 脳 10^鈭? and 9 脳 10^鈭? M creatinine concentration in phosphate buffer solution at pH 7.20. The biosensor exhibited good operational and storage stability for at least 4 weeks kept in dry at 4-6 掳C. It had a reproducible and stable response during continuous work at least for 10 h with the relative standard deviation of 0.5%(n = 48) for creatinine of 1 脳 10^鈭? M in phosphate buffer solution.