Gastroprotective potential of frutalin, a d-galactose binding lectin, against ethanol-induced gastric lesions

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• 作者：Ana Paula de Vasconcellos Abdon^a ; Greicy Coelho de Souza^a ; Lí ; lian Noronha Coelho de Souza^a ; Renata Prado Vasconcelos^a ; Carolina Araú ; jo Castro^a ; Marjorie Moreira Guedes^b ; Roberto Cé ; sar Pereira Lima Jú ; nior^b ; Renato de Azevedo Moreira^a ; Ana Cristina de Oliveira Monteiro-Moreira^a ; Adriana Rolim Campos^a ; ^{adrirolim@unifor.br}
• 关键词：Artocarpus incisa ; Frutalin ; Gastroprotection ; Absolute ethanol
• 刊名：Fitoterapia
• 出版年：2012
• 期刊代码：35_0367326x
• 类别：ch
• 出版时间：April, 2012
• 卷：83
• 期：3
• 页码：604-608
• 文件大小：174 K

摘要

The present study was designed to verify whether frutalin (FTL) affords gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2 ml of ethanol (96%). Mice in groups were pretreated with FTL (0.25, 0.5 and 1 mg/kg; i.p.), cimetidine (100 mg/kg; p.o.), or vehicle (0.9%of NaCl, 10 mL/kg; p.o.), 30 min before ethanol administration. They were sacrificed 30 min later, the stomachs excised, and the mucosal lesion area (mm²) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide, sulphydryls, ATP-sensitive potassium channels, adrenoceptors, opioid receptors and calcium channels were analyzed. Treatments effects on ethanol-associated oxidative stress markers GSH and MDA were measured in gastric tissue. FTL afforded a dose-unrelated gastroprotection against the ethanol damage. However, it failed to prevent the ethanol-induced changes in the levels of GSH and MDA. It was observed that the gastroprotection by FTL was greatly reduced in animals pretreated with capsazepine, indomethacin, L-NAME or glibenclamide. Considering the results, it is suggested that the FTL could probably be a good therapeutic agent for the development of new medicine for the treatment of gastric ulcer.