- 作者：Marcelo Ferraz Sampaio ; Mario Hiroyuki Hirata ; Rosario Dominguez Crespo Hirata ; Fabiana Cristina Pereira Santos ; Raffaella Picciotti ; André ; Ducati Luchessi ; Sonia de Quateli Doi ; Dikran Armaganij
- 关键词：Acute myocardium infarction ; Gene polymorphism ; Endothelial nitric oxide synthase ; Fibrinogen ; Plasminogen activator inhibitor-1 ; Endothelium function
- 刊名：Clinica Chimica Acta
- 出版年：2007
- 期刊代码：54_00098981
- 类别：med
- 出版时间：2 February 2007
- 卷：377
- 期：1-2
- 页码：154-162
- 文件大小：205 K
We investigated the relationship between NOS3, FGB and PAI-1 polymorphisms and endothelial dysfunction and risk factors for acute myocardial infarction (AMI) in young adults.
Methods
Endothelial function was measured by response to flow mediated vasodilation (FMV) and induced by nitrate (FMN). Biochemical parameters were measured by standard enzymatic methods and plasma total nitrate was analyzed by the NOA™ system. NOS3 (T-786C, G894T and intron 4A/B STR), FGB (C-148T and G-455A) and PAI-1 (4G/5G) polymorphisms were determined by PCR-RFLP.
Results
Concentrations of total and LDL cholesterol, apo B, triglycerides, nitrate, PAI-1 and fibrinogen were higher and apo AI, HDL cholesterol and FMV were lower in AMI patients than in controls (p < 0.001). PAI-1 (p < 0.001) but not nitrate was higher in AMI patients with low response to FMV. NOS3 T-786C and FGB C-148T polymorphisms were associated with AMI (p < 0.050). NOS3 T-786C was also related to hypertension (p = 0.049). NOS3 intron 4A/B STR was associated with increased concentrations of total cholesterol and apo B. NOS3-786TT/894GT haplotype was associated with increased FMV (p = 0.018) than the other haplotypes.
Conclusions
Our data suggest NOS3 and FGB polymorphisms are associated with AMI. NOS3 is also related to hypertension, endothelial dysfunction and variation on serum cholesterol in young adults with AMI.