Protective effect of Chresta martii extract on ethanol-induced gastropathy depends on alpha-2 adrenoceptors pathways but not on nitric oxide, prostaglandins or opioids

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• 作者：Antonio Alfredo Rodrigues e Silva^a ; ^{alfredodentista@gmail.com} ; Mirna Marques Bezerra^a ; ^{mirnabrayner@gmail.com} ; Hellí ; ada Vasconcelos Chaves^a ; ^{helliadachaves@yahoo.com.br} ; Vicente de Paulo Teixeira Pinto^b ; ^{pintovicente@gmail.com} ; Eryvelton de Souza Franco^c ; ^{eryvelton_franco@hotmail.com} ; &Acirc ; ngela Magalh&atilde ; es Vieira^a ; ^{angelamvet@hotmail.com} ; Edeline Barbosa Araú ; jo^a ; ^{edeline_araujo@hotmail.com} ; Lí ; via Cunha Rios^a ; ^{liviacunharios@hotmail.com} ; Ana Catarina Resende Leite^c ; ^{anacatarinar@gmail.com} ; Maria Bernadete de Sousa Maia¹ ; ^c ; ^{maria.maia@pq.cnpq.br}
• 关键词：Chresta ; Gastropathy ; Alpha-2 adrenoceptors
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：26 June, 2012
• 卷：142
• 期：1
• 页码：206-212
• 文件大小：878 K

摘要

lass="h4">Ethnopharmacological relevance

Species of Chresta genus- are recognized by the population of northeastern Brazil as traditional herbs used to treat gastric diseases and other disorders.

lass="h4">Aim of the study

This work aimed to find out the action mechanism of Chresta martii hydro alcoholic extract gastro protective effect in the model of ethanol-induced gastropathy.

lass="h4">Material and methods

Gastropathy was assessed by percentual damaged area determination in photographs of mice opened stomachs. Fasted mice treated with ethanol 99.9%(0.2 ml/animal, p.o.) were pre-treated with Chresta martii hydro alcoholic extract (HAE) (50, 100 or 200 mg/kg, p.o.), ranitidine (80 mg/kg, p.o.) or saline (5 ml/kg; p.o.) in different experimental sets, in which pharmacological tools (naloxone, indomethacin, N_蠅-Nitro-L-arginine methyl ester hydrochloride (L-NAME) or yohimbine) were added in order to clarify a possible action mechanism. Animals were sacrificed 30 min after ethanol challenge to stomach analysis. Determination of non-protein sulfhydryl groups and tissue hemoglobin, besides histological assessment (H&E) were taken to fully characterize the HAE gastro protective effect.

lass="h4">Results

HAE (100 and 200 mg/kg) was able to protect mucosa against ethanol gastropathy in presence of three (naloxone, indomethacin and L-NAME) of four antagonist/inhibitor tools. The HAE effect was reversed only by yohimbine, showing the alpha-2 adrenoceptors participation on gastro protective effect of this extract. HAE histological characteristics, NP-SH and Hb were compatible with the protective effects.

lass="h4">Conclusions

HAE possesses gastroprotective effects in an ethanol-induced gastropathy model in mice, corroborating the traditional use of this family of plants to treat gastric disorders. This activity is mediated by alpha-2 adrenoceptors activation, but not by nitric oxide release, opioid receptor activation or prostaglandin synthesis. HAE also has antioxidant activity that is thought to either play a role in this biological activity or to be a byproduct of alpha-2 adrenergic complex activation.