Redox active molecules cytochrome c and vitamin C enhance heme-enzyme peroxidations by serving as non-specific agents for redox relay

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• 作者：Sudeep Kumar Gade ; Subarna Bhattacharya¹ ; Kelath Murali Manoj ; ^{satyamjayatu@yahoo.com} ; ^{muralimanoj@vit.ac.in}
• 关键词：ABTS ; 2 ; 2&prime ; -azino-bis(3-ethylbenzothiazoline-6-sulfonate) disodium salt ; CPO ; chloroperoxidase ; CPR ; cytochrome P450 reductase ; CYP ; cytochrome P450 ; cyt. b5 ; cytochrome b5 ; cyt. c ; cytochrome c ; DROS ; diffusible reactive/reduced oxygen species ; Gua
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：9 March, 2012
• 卷：419
• 期：2
• 页码：211-214
• 文件大小：392 K

摘要

We report that incorporation of very low concentrations of redox protein cytochrome c and redox active small molecule vitamin C impacted the outcome of one-electron oxidations mediated by structurally distinct plant/fungal heme peroxidases. Evidence suggests that cytochrome c and vitamin C function as a redox relay for diffusible reduced oxygen species in the reaction system, without invoking specific or affinity-based molecular interactions for electron transfers. The findings provide novel perspectives to understanding - (1) the promiscuous role of cytochrome b₅ in the metabolism mediated by liver microsomal xenobiotic metabolizing systems and (2) the roles of antioxidant molecules in affording relief from oxidative stress.