IL-17A promotes the growth of airway epithelial cells through ERK-dependent signaling pathway
- 作者：Daisuke Inoue ; Muneo Numasaki ; Mika Watanabe ; Hiroshi Kubo ; Takahiko Sasaki ; Hiroyasu Yasuda ; Mutsuo Yamaya and Hidetada Sasaki
- 关键词：IL-17A ; Mucin ; Airway epithelial cells ; and mitogen-activated protein kinase kinase
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：8 September 2006
- 卷：347
- 期：4
- 页码：852-858
- 文件大小：247 K
摘要
The effects of IL-17A on mucin production and growth of airway epithelial cells were examined. Histological and immunohistochemical analyses revealed that IL-17A increased the mucin production and number of tracheal epithelial cells in air–liquid interface cultures. The biological property of IL-17A to stimulate the mucin production by tracheal epithelial cells was determined using an ELISA. The mitogenic effect of IL-17A on tracheal epithelial cells was confirmed with Calcein-AM assay. The growth-stimulatory effect of IL-17A was dose-dependent and mediated via the ERK MAP kinase pathway. Inhibitors of MEK abrogated the mitogenic effect of IL-17A, whereas an inhibitor of p38 or JNK displayed no significant inhibitory effect. Moreover, relatively lower doses of IL-13 also significantly increased the growth of tracheal epithelial cells through a distinct signaling pathway from that of IL-17A. These findings provide the first evidence that IL-17A stimulates the growth of airway epithelial cells through the ERK MAP kinase pathway.