Ovariectomy increases neuronal amyloid-β binding alcohol dehydrogenase level in the mouse hippocampus

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• 作者：Emiko Fukuzaki ; Kazuhiro Takuma ; Yoko Funatsu ; Yukiko Himeno ; Yuko Kitahara ; Bin Gu ; Hiroyuki Mizoguchi ; Daisuke Ibi ; Koji Koike ; Masaki Inoue ; Shi Du Yan ; Kiyofumi Yamada
• 关键词：Ovariectomy ; Alzheimer's disease ; β ; -Amyloid ; ABAD ; RAGE
• 刊名：Neurochemistry International
• 出版年：2008
• 期刊代码：120_01970186
• 类别：bio
• 出版时间：June 2008
• 卷：52
• 期：7
• 页码：1358-1364
• 文件大小：946 K

摘要

Ovarian hormone decline after menopause may influence cognitive performance and increase the risk for Alzheimer's disease (AD) in women. Amyloid-β peptide (Aβ) has been proposed to be the primary cause of AD. In this study, we examined whether ovariectomy (OVX) could affect the levels of cofactors Aβ-binding alcohol dehydrogenase (ABAD) and receptor for advanced glycation endproducts (RAGE), which have been reported to potentiate Aβ-mediated neuronal perturbation, in mouse hippocampus, correlating with estrogen and Aβ levels. Female ICR mice were randomly divided into ovariectomized or sham-operated groups, and biochemical analyses were carried out at 5 weeks after the operation. OVX for 5 weeks significantly decreased hippocampal 17β-estradiol level, while it tended to reduce the hormone level in serum, compared with the sham-operated control. In contrast, OVX did not affect hippocampal Aβ_1-40 level, although it significantly increased serum Aβ_1-40 level. Furthermore, we demonstrated that OVX increased hippocampal ABAD level in neurons, but not astrocytes, while it did not affect RAGE level. These findings suggest that the expression of neuronal ABAD depends on estrogen level in the hippocampus and the increase in serum Aβ and hippocampal ABAD induced by ovarian hormone decline may be associated with pre-stage of memory deficit in postmenopausal women and Aβ-mediated AD pathology.