CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin α5β1
- 作者：Mitsuhiro Hoshijima ; Takako Hattori ; Miho Inoue ; Daisuke Araki ; Hiroshi Hanagata ; Akira Miyauchi and Masaharu Takigawa
- 关键词：CCN family ; CCN family protein 2/connective tissue growth factor ; Binding protein ; Fibronectin ; Integrin ; α ; vβ ; 3 ; α ; 5β ; 1 ; Cell adhesion
- 刊名：FEBS Letters
- 出版年：2006
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：20 February 2006
- 卷：580
- 期：5
- 页码：1376-1382
- 文件大小：1034 K
摘要
Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against α5β1 integrin (α5β1), but not by one against anti-αvβ3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that α5β1 is involved in this adhesion.