Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase—Application to analysis of triamcinolone acetonide

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• 作者：Lucie Havlí ; ková ; Ludmila Matysová ; Renata Há ; jková ; Dalibor &#x160 ; atí ; nský ; Petr Solich
• 关键词：Degradation product ; C18 columns ; HPLC ; Pentafluorophenylpropyl stationary phase ; Triamcinolone acetonide
• 刊名：Talanta
• 出版年：2008
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：30 July 2008
• 卷：76
• 期：3
• 页码：597-601
• 文件大小：326 K

摘要

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using estradiol hemihydrate as an internal standard, has been developed and validated. Chromatography was performed on a Discovery HS F5 column (150 mm × 4.6 mm, 5 μm) using a binary mobile phase composed of acetonitrile and water 45:55 (v:v). The flow-rate was 0.6 mL/min, the column temperature 25 °C and the UV detection was accomplished at 240 nm.