Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: A follow-up to an ECVAM workshop
- 作者：David Kirkl ; Peter Kasper ; Lutz Mü ; ller ; Raffaella Corvi ; Gü ; nter Speit
- 关键词：Genotoxicity in vitro ; Reliability ; Improved tests ; New tests ; Recommended chemicals
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2008
- 期刊代码：31_13835718
- 类别：pha
- 出版时间：31 May 2008
- 卷：653
- 期：1-2
- 页码：99-108
- 文件大小：225 K
摘要
At a recent ECVAM workshop considering ways to reduce the frequency of irrelevant positive results in mammalian cell genotoxicity tests [D. Kirkland, S. Pfuhler, D. Tweats, M. Aardema, R. Corvi, F. Darroudi, A. Elhajouji, H.-R. Glatt, P. Hastwell, M. Hayashi, P. Kasper, S. Kirchner, A. Lynch, D. Marzin, D. Maurici, J.-R. Meunier, L. Müller, G. Nohynek, J. Parry, E. Parry, V. Thybaud, R. Tice, J. van Benthem, P. Vanparys, P. White, How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary followup animal tests: Report of an ECVAM Workshop, Mutat. Res. 628 (2007) 31–55], recommendations for improvements/modifications to existing tests, and suggestions for new assays were made. Following on from this, it was important to identify chemicals that could be used in the evaluation of modified or new assays. An expert panel was therefore convened and recommendations made for chemicals to fit three different sets of characteristics, namely:
- Group 1: Chemicals that should be detected as positive in in vitro mammalian cell genotoxicity tests. Chemicals in this group are all in vivo genotoxins, either due to DNA-reactive or non DNA-reactive mechanisms (e.g., induction of aneuploidy, inhibition of topoisomerase). Most of them are also known carcinogens with a mutagenic mode of action.