Deletion of the single putative N-glycosylation site of the porcine circovirus type 2 Cap protein enhances specific immune responses by DNA immunisation in mice

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• 作者：Jinyan Gu¹ ; Ruibin Cao¹ ; Yu Zhang ; Xue Lian ; Hassan Ishag ; Puyan Chen ; ^{puyanchen@yahoo.com.cn}
• 关键词：Porcine circovirus type 2 ; Cap protein ; N-linked glycosylation ; Immune response ; DNA vaccines
• 刊名：The Veterinary Journal
• 出版年：2012
• 期刊代码：338_10900233
• 类别：med
• 出版时间：June, 2012
• 卷：192
• 期：3
• 页码：385-389
• 文件大小：552 K

摘要

The immunogenicity of a putative N-linked glycosylation site located at amino acids 143-145 (N143YS) of the porcine circovirus 2 (PCV2) Cap protein was investigated. Eukaryotic vectors expressing wild-type PCV2 Cap (pCap) and N-linked glycosylation site mutant Cap (pCap-m) were constructed and the immunogenicity of these proteins was determined following DNA vaccination in BALB/c mice. pCap-m elicited significantly higher Cap-specific T lymphocyte proliferative activity, percentage of CD8⁺ T cells, ratio of immunoglobulin (Ig) G2a:IgG1 and levels of interferon-纬 compared to pCap (P < 0.05). These results indicate that deletion of the N-glycosylation site in the PCV2 Cap protein enhances specific immune responses and may have a role in Cap-based DNA vaccines with enhanced immunogenicity.