In vitro effect of free and complexed indium(III) against Mycobacterium tuberculosis
- 作者：Suzana David ; Vanessa Barros ; Carla Cruz and Rita Delgado
- 关键词：Mycobacterium tuberculosis ; Macrocyclic compounds ; Metal complexes
- 刊名：FEMS Microbiology Letters
- 出版年：2005
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：2005
- 卷：251
- 期：1
- 页码：119-124
- 文件大小：132 K
摘要
In mycobacteria, the study of inhibition by metal ions has been limited by the absence of suitable molecular vectors. Recently, we reported on the inhibitory activity of a family of chelators, macrocyclic compounds (MCC), against Mycobacterium tuberculosis. In this study equimolar concentrations of the free cations vanadium(IV), arsenic(III), iron(III), indium(III) and bismuth(III), and as 1:1 complexes with the MCC 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetra-acetic acid (TETA) were tested in vitro against M. tuberculosis using the Bactec™ 460 TB radiometric technology (Becton-Dickinson, MD, USA). Radiometric inhibition above 80%was obtained with free indium(III) and bismuth(III), and ranged from 80%to 99%, with the complexes of TETA with vanadium(IV), bismuth(III) and indium(III), in the order of increasing activity. The highest radiometric inhibition levels were obtained with the [In(TETA)]− complex, which caused drops of up to 4 log units in cellular viability. The minimal inhibitory concentration of this compound was evaluated at 3 μM.