Effect of polyamidoamine dendrimer G3 and G4 on skin permeation of 8-methoxypsoralene鈥擨n vivo study

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• 作者：Katarzyna Borowska^a ; Stanisł ; aw Woł ; owiec^a ; ^{swolowiec@wsi.rzeszow.pl} ; Andrzej Rubaj^b ; Kazimierz Gł ; owniak^c ; Elwira Sieniawska^c ; Sebastian Radej^d
• 关键词：PAMAM dendrimers ; 8-MOP ; PUVA ; Rat skin permeation ; Confocal microscopy
• 刊名：International Journal of Pharmaceutics
• 出版年：2012
• 期刊代码：24_03785173
• 类别：pha
• 出版时间：15 April, 2012
• 卷：426
• 期：1-2
• 页码：280-283
• 文件大小：542 K

摘要

In the present study we have assessed the ability of (PAMAM) dendrimers G3 and G4 to facilitate transdermal delivery of 8-methoxypsoralen (8-MOP) in vivo. In vitro study using Franz diffusion cell revealed an enhanced transdermal flux for 8-MOP in complex with G3 and G4 dendrimer in relation to standard 8-MOP solution. In present study in vivo skin permeation potential of 8-MOP complex with G3 and G4 PAMAM dendrimer was assessed using confocal laser scanning microscopy (CLSM), which revealed an enhanced permeation of the 8-MOP to the deeper layers of the skin and significantly higher concentration in comparison with standard 8-MOP solution. Skin tissue 8-MOP concentration, evaluated by HPLC indicates that G3 and G4 PAMAM application significantly increase 8-MOP skin deposition in comparison with standard 8-MOP solutions after 1 and 2 h. G4 appeared to be a more effective 8-MOP penetration enhancer than G3 PAMAM. Our results suggest the feasibility of G3 and G4 PAMAM dendrimers for transdermal delivery of 8-MOP resulting in better skin permeation and higher concentration of 8-MOP in epidermis and dermis of the drug that could help to improve effectiveness and safety of PUVA therapy.