Fluorescence competition assay for the assessment of green leaf volatiles and trans-尾-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius)
- 作者：Tao Zhong1 ; zhongtao_069@163.com ; Jiao Yin1 ; ajiaozi@163.com ; Sisi Deng gracessdeng@gmail.com ; Kebin Li likebin54@163.com ; Yazhong Cao ; yzcao@ippcaas.cn
- 关键词：Fluorescence competition assay ; Green leaf volatiles ; trans-尾-farnesene ; Odorant-binding proteins ; Sitobion avenae
- 刊名：Journal of Insect Physiology
- 出版年：2012
- 期刊代码：92_00221910
- 类别：bio
- 出版时间：June, 2012
- 卷：58
- 期：6
- 页码：771-781
- 文件大小：1677 K
摘要
Odorant-binding proteins (OBPs) are important parts of insect olfactory systems, and sensitive olfaction is vital for phytophagous insects in host foraging. Electrophysiological studies are helpful in understanding olfactory sensing in Sitobion avenae (Hemiptera: Aphididae), but the functions of odorant-binding proteins in this insect are poorly understood. In this study, we used fluorescence competition assays to measure the binding specificities of SaveOBPs. The results showed that both SaveOBP2 and SaveOBP3 were superior to SaveOBP7 in binding green leaf volatiles. It was unexpected that SaveOBP7 bound trans-尾-farnesene strongly, which was known as alarm pheromone of this species. Host volatiles were recognized much more easily by SaveOBP2, and the observed binding activity of SaveOBP2 equaled for tested green leaf volatiles. Our results imply that SaveOBP7 might play a more important role in aphid alarm pheromone discrimination.