Estrogen plays an important role in male reproduction. Most of the actions are mediated by estrogen receptor (ER). To investigate the profile of estrogen affecting male fertility, we firstly cloned four ERs from the male
Spinibarbus denticulatus, a local economically important cyprinid fish in China. Phylogenetic tree analysis ranked the four sdERs as two distinct groups of ER
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and β, which could be further divided into duplicated isoforms 1 and 2, respectively. High score identities were shared between each of the duplicated isoforms. All of the four sdERs distributed in central nervous system of male fish with a quite broad spectrum. However, distribution diversity became evident between sdER
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and sdERβ subtypes in the peripheral tissues. Both of the two isoforms of ERβ were detected in all seven tissues examined, while expression of sdER
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1 was mainly limited to liver, kidney, testis and intestine and sdER
![greek small letter alpha greek small letter alpha](http://www.sciencedirect.com/scidirimg/entities/204e.gif)
2 was confined to liver, heart, kidney, testis and gill. During the testis recrudescing stages, serum concentration of luteinizing hormone (LH), testosterone (T) and estradiol-17β (E
2) were increasing. T and LH levels in the circulation were high until the later fully recrudesced phase, while serum E
2 level was low all the time. Quantitative real-time RT-PCR analysis determined the most abundance of sdERs in pituitary where the two sdER
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isoforms positively expressed with testis development, while sdERβ isoforms expressed with a reverse pattern. sdER
![greek small letter alpha greek small letter alpha](http://www.sciencedirect.com/scidirimg/entities/204e.gif)
1 and sdERβ1 were the primary forms in testis. sdER
![greek small letter alpha greek small letter alpha](http://www.sciencedirect.com/scidirimg/entities/204e.gif)
1 gradually increased during the recrudescence process while sdERβ1 firstly decreased during the recrudescing stage and then positively expressed in fully recrudesced stage. Little or no signal was detected in brain. The present work provided evidence of four sdERs in male reproductive system and suggested an important role of sdER
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1 during testis recrudescence. Pituitary contained duplicates forms of sdER
![greek small letter alpha greek small letter alpha](http://www.sciencedirect.com/scidirimg/entities/204e.gif)
which may play a role in the feedback effects of estrogen on LH secretion.