Regulation of the antigenomic delta ribozyme catalytic activity by complexes of triazole derivatives with transition metal ions in a pH-dependent manner

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• 作者：Joanna Zeidler ; Danuta Nijakowska ; Jan Wrzesinski ; ^{wrzesinj@ibch.poznan.pl}
• 关键词：Triazole derivatives&ndash ; metal ion complexes ; HDV ribozyme ; pH dependence
• 刊名：Inorganic Chemistry Communications
• 出版年：2012
• 期刊代码：39_13877003
• 类别：ch
• 出版时间：June, 2012
• 卷：20
• 期：Complete
• 页码：330-333
• 文件大小：293 K

摘要

The effect of several triazole compounds (1,2,4-triazole and its 3-ethoxycarbonyl derivative as well as 1,2,3-triazole, its 4-[N-methyl(2,2,2-trifluoroacetamide)] derivative and two 1,2,3-triazole ribonucleosides) and their complexes with transition metal ions (Co^2 +, Ni^2 +, Zn^2 + Cd^2 + and Cu^2 +) on the catalytic activity of antigenomic HDV ribozyme was tested at different pH values. Investigated at pH 7.5, 1,2,3- and 1,2,4-triazole derivatives only slightly inhibited Mg^2 +-induced catalytic cleavage of the ribozyme. In comparison, Co^2 + and Ni^2 + complexes of 1,2,3-triazole and their derivatives, namely, N-(1,2,3-triazol-4-yl)methyl-2,2,2-trifluoroacetamide and 1-尾-d-ribofuranosyl-1H-1,2,3-triazole-4-carboxylic acid amide inhibited the cleavage reaction 4-5-fold more strongly.

Additionally, the application of Zn^2 + complexes of 1,2,3- and 1,2,4-triazole instead of catalytic Mg^2 + ion, promoted the cleavage activity of the ribozyme at pH 5.5. The 1,2,3-triazole-Zn^2 + complex was found to be the most effective.