Determination of intracellular pH using sensitive, clickable fluorescent probes

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• 作者：Nazmiye B. Yapici^a ; Srinivas Rao Mandalapu^a ; Teng-Leong Chew^b ; Satya Khuon^b ; Lanrong Bi^a ; ^{lanrong@mtu.edu}
• 关键词：Intracellular pH ; Fluorescent probe
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 April, 2012
• 卷：22
• 期：7
• 页码：2440-2443
• 文件大小：569 K

摘要

We synthesized and evaluated a series of acidic fluorescent pH probes exhibiting robust pH dependence, high sensitivity and photostability, and excellent cell membrane permeability. Titration analyses indicated that probe 3 could increase its fluorescence intensity 800-fold between pH 8.0 and 4.1. Additionally, its pK_a value is optimal for intracellular probing of acidic organelles. Fluorescent imaging of HepG2 and Hela cells further revealed that probe 3 demonstrates outstanding capacity for monitoring of intracellular [H⁺] levels. The easily accessible terminal alkyne/azido function groups of these probes offer the possibility of rapidly constructing sensor molecule libraries using 鈥榗lick鈥?chemistry.