The study of interferences for diagnosing albuminuria by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry

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• 作者：Yi-Tzu Cho^a ; ^b ; Yu-Syuan Chen^a ; Jia-Lun Hu^a ; Jentaie Shiea^a ; ^c ; ^d ; ^{jetea@mail.nsysu.edu.tw} ; Shih Meng Yeh^e ; Hung-Chun Chen^e ; Yi-Chern Lee^f ; Deng-Chyang Wu^c ; ^d ; ^f
• 关键词：Albuminuria ; Urinary dipstick ; Interferences ; Matrix-assisted laser desorption ionization ; Albumin ; Mass spectrometry
• 刊名：Clinica Chimica Acta
• 出版年：2012
• 期刊代码：54_00098981
• 类别：med
• 出版时间：18 May, 2012
• 卷：413
• 期：9-10
• 页码：875-882
• 文件大小：728 K

摘要

Background

Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometric analysis of albumin (ALB) biomarkers is an alternative approach toward the rapid diagnosis of proteinuria for screening a large number of samples. The aim of this study is to reveal if interfering factors in urinary dipstick approach would affect the results of diagnosing albuminuria by MALDI-TOF MS.

Methods

The effects of various interfering chemicals on the diagnosis of albumin in urine were examined using both MALDI-TOF mass spectrometric and dipstick approaches. Semi-quantification of albumin was performed by using MALDI-TOF MS.

Results

Interferences from various drugs, detergents, vitamins and their metabolites, alkaline, and blood, which often cause false-positive and false-negative results in conventional urinary dipstick analysis, are avoided when using this MALDI-TOF MS approach. It was found that the intensity of + 1 and + 2 albumin ions varies with the albumin concentration. A log/log plot of the intensity ratio vs. albumin concentration is then used as a calibration curve for semi-quantifying the albumin in urines.

Conclusions

MALDI-TOF MS is an effective approach toward avoiding interferences caused by various chemical compounds during the rapid diagnosis of albumin in urine. Semi-quantification of albuminuria is also achieved by this MALDI-TOF MS approach.