Diagnostic serum proteomic analysis in patients with active tuberculosis

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• 作者：Junxian Zhang^a ; Xueqiong Wu^a ; ^{wu-xueqiong@263.net} ; Lilan Shi^b ; Yan Liang^a ; Zhensheng Xie^b ; Yourong Yang^a ; Zhongxing Li^c ; Cuihua Liu^a ; Fuchuan Yang^b
• 关键词：TB ; Tuberculosis ; SELDI/TOF-MS ; the surface-enhanced laser desorption ionization time of flight mass spectrometry ; HPLC ; high performance liquid chromatography ; MALDI-TOF-MS ; tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrome
• 刊名：Clinica Chimica Acta
• 出版年：2012
• 期刊代码：54_00098981
• 类别：med
• 出版时间：18 May, 2012
• 卷：413
• 期：9-10
• 页码：883-887
• 文件大小：318 K

摘要

Background

The diagnosis for smear-negative pulmonary tuberculosis (TB) is very difficult. Proteomic fingerprinting of sera is a potentially useful tool.

Methods

This study analyzed the results of the proteomic fingerprinting in sera obtained from active TB patients and controls using the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and protein-chip technology. The peaks were detected and analyzed, and a diagnostic system was developed. The protein peak was identified using high performance liquid chromatography (HPLC)-tandem matrix-assisted laser desorption/ionization-TOF-MS (MALDI-TOF-MS).

Results

Around 50 protein peaks were found significantly different between the TB patients and the controls (P < 0.01). Three protein peaks at m/z 5643, 4486 and 4360 were selected for system classification and the development of a decision model. The model distinguished the TB patients from the controls with a sensitivity of 96.9%and a specificity of 97.8%, respectively. The diagnostic accuracy was up to 97.3%. The one most discrepant protein peak at m/z 5643 seen in sera of active TB patients was identified as orosomucoid.

Conclusions

A diagnostic system for active TB was developed using mass spectrometry and protein chip technology and required only small-volume serum samples. One potential protein biomarker at m/z 5643 was identified as orosomucoid.