A validated capillary electrophoresis method for simultaneous determination of ezetimibe and atorvastatin in pharmaceutical formulations
- 作者：Mona M. AlShehri ; malshihri@ksu.edu.sa ; abs.office1@yahoo.com
- 关键词：Ezetimibe ; Atorvastatin ; Capillary electrophoresis ; Diode array detector ; Tablets
- 刊名：Journal of the Saudi Pharmaceutical Society
- 出版年：2012
- 期刊代码：P131_13190164
- 类别：pha
- 出版时间：April, 2012
- 卷：20
- 期：2
- 页码：143-148
- 文件大小：435 K
摘要
A simple, precise, and sensitive capillary electrophoresis technique coupled with a diode array detector has been developed for the separation and simultaneous determination of ezetimibe and atorvastatin in pharmaceutical formulations. Separation of both ezetimibe and atorvastatin was achieved utilizing fused silica capillary (58 cm 脳 75 渭m ID) and background electrolyte solution that consisted of phosphate buffer (2.5 mM, pH 6.7): methanol (70:30 v/v). The proposed method was validated by testing its specificity, linearity, precision, accuracy, recovery, and detection limit/quantitation limit values. The method was linear over the range 2.5-50 渭g/ml for ezetimibe (r = 0.9992) and 1-100 渭g/ml for atorvastatin (r = 0.9999). Within-day and between-day RSD for ezetimibe and atorvastatin were 猢?.6%and 猢?.9%, respectively. The detection limit was 0.07 渭g/ml for ezetimibe and 0.06 渭g/ml for atorvastatin. The validated method was successfully employed for the determination of ezetimibe and atorvastatin in tablets with no interfering peaks from common pharmaceutical excipients. The percentage recoveries of the two drugs from their tablets were 99.80 卤 1.76 and 100.19 卤 1.83, respectively.