Development of a monoclonal antibody-based competitive ELISA for detection of β-conglycinin, an allergen from soybean

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• 作者：Jinming You ; Defa Li ; Shiyan Qiao ; Zirui Wang ; Pingli He ; Deyuan Ou ; Bing Dong
• 关键词：Soybean allergen ; β ; -Conglycinin ; Immunoassay ; Monoclonal antibody
• 刊名：Food Chemistry
• 出版年：2008
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：1 January 2008
• 卷：106
• 期：1
• 页码：352-360
• 文件大小：204 K

摘要

Soybean β-conglycinin is one of the major food allergies for children and young animals. In order to detect immunoreactive β-conglycinin from soybean and soybean products, monoclonal antibodies against β-conglycinin were prepared using a conjugated chicken ovalbumin with a synthetic peptide that corresponded to one epitope sequence of β-conglycinin as the immunogen. The generated monoclonal antibodies, named as 6G₄, 3B₇, and 5F₁₀, were identified as being of IgG₁ isotype and exhibited high specificity to β-conglycinin. A competitive enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody 6G₄ was established to determine β-conglycinin and showed an IC₅₀ value of 4.7 ng/mL with a detection limit of 2.0 ng/mL. The recovery tests of β-conglycinin indicated that the monoclonal antibody 6G₄-based competitive ELISA gave reliable reproducibility. Therefore, the produced monoclonal antibody 6G₄ and the developed competitive ELISA could provide a valuable tool for sensitive determination of β-conglycinin and for future studies on food allergies related to soybean β-conglycinin.