Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS

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• 作者：Chia-Ching Chen^a ; ¹ ; Ying-Chieh Lu^b ; ¹ ; Yi-Wen Chen^b ; ¹ ; Wen-Li Lee^a ; Chieh-Hsiang Lu^c ; You-Hsuan Chen^b ; Yun-Ching Lee^b ; Szu-Ting Lin^b ; John F. Timms^d ; ^{john.timms@ucl.ac.uk} ; Ying-Ray Lee^e ; ^f ; ^{yingray.lee@gmail.com} ; Hsiu-Chuan Chou^g ; ^{chouhc@mail.nhcue.edu.tw} ; Hong-Lin Chan^b ; ^{hlchan@life.nthu.edu.tw}
• 关键词：2-DE ; two-dimensional gel electrophoresis ; CCB ; colloidal Coomassie Blue ; CHAPS ; 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate) ; ddH2O ; double deionized water ; DIGE ; differential gel electrophoresis ; DTT ; dithiothreitol ; MALDI&ndash ; TOF MS ; m
• 刊名：Journal of Proteomics
• 出版年：2012
• 期刊代码：P37_18743919
• 类别：ch
• 出版时间：27 June, 2012
• 卷：75
• 期：12
• 页码：3760-3777
• 文件大小：1911 K

摘要

Type 1 diabetes mellitus (T1DM) is an insulin-dependent metabolic disease in the world and often occurs in children and adolescents. Recent advances in quantitative proteomics offer potential for the discovery of plasma proteins as biomarkers for tracking disease progression and for understanding the molecular mechanisms of diabetes. Comparative proteomic analysis of the plasma proteomes from T1DM cases and healthy donors with lysine- and cysteine-labeling 2D-DIGE combining MALDI-TOF/TOF mass spectrometry revealed that 39 identified T1DM-associated plasma proteins showed significant changes in protein expression including hemopexin, and 41 in thiol reactivity. Further study showed that hemopexin can be induced in numerous cell lines by increasing the glucose concentration in the medium. Interestingly, glucose-induced hemopexin expression can be reduced by reactive oxygen species (ROS) scavengers such as glutathione, implying that hemopexin expression is linked to glucose-induced oxidative stress. In conclusion, the current work has identified potential T1DM biomarkers and one of these, hemopexin, can be modulated by glucose through a ROS-dependent mechanism.