Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco

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• 关键词：Oligochitosan ; Tobacco mosaic virus ; Plant protection ; Nitric oxide ; Hydrogen peroxide
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2007
• 期刊代码：182_00483575
• 类别：bio
• 出版时间：January 2007
• 卷：87
• 期：1
• 页码：78-84
• 文件大小：670 K

摘要

Oligochitosan was applied by spraying it on tobacco leaves for inhibition of tobacco mosaic virus (TMV). The maximum inhibition of TMV by oligochitosan was observed when inoculation occurred at 24 h after spraying 50 μg ml⁻¹ oligochitosan. The production of H₂O₂ and NO in epidermal tobacco cells induced by oligochitosan was investigated by epidermal strip bioassay and LSCM, using cell permeable fluorophore diaminofluorescein diacetate (DAF-2D) and 2′,7′-dichlorofluorescin diacetate (H₂DCF-DA), respectively. Epidermal tobacco cells treated with oligochitosan resulted in a strong increase of intracellular NO and H₂O₂. Oligochitosan and NO donor sodium nitroprusside (SNP) induced the defense reaction against tobacco mosaic virus (TMV), and increased phenylalanine ammonia-lyase (PAL) activity. Co-treatment of the tobacco cells with oligochitosan and NO scavenger CPTIO blocked the inducing resistance. The results indicated that the defense response induced by oligochitosan was connected with NO pathway.