A proteome analysis of Corynebacterium glutamicum after exposure to the herbicide 2,4-dichlorophenoxy acetic acid (2,4-D)
- 作者：Ali Fanous ; Florian Weil ; Carsten Lü ; ck ; Angelika Gö ; rg ; Albrecht Friess ; Harun Parlar
- 关键词：Corynebacterium glutamicum ; 2 ; 4-dichlorophenoxy acetic acid ; Stress response ; Matrix assisted laser desorption ; Ionization time-of-flight mass spectrometry ; Two-dimensional gel electrophoresis
- 刊名：Chemosphere
- 出版年：2007
- 期刊代码：15_00456535
- 类别：env
- 出版时间：August 2007
- 卷：69
- 期：1
- 页码：25-31
- 文件大小：562 K
摘要
The herbicide 2,4-dichlorophenoxy acetic acid (2,4-D) induces a wide spectrum of toxic responses in living organisms. In this study, we analyzed the stress-induced responses of Corynebacterium glutamicum cells on protein level upon treatment with 2,4-D. For this, growing C. glutamicum cells were exposed to sublethal concentrations of 2,4-D, and changes of the gene expression profiles in comparison to non-exposed organisms were analyzed by two-dimensional gel electrophoresis and mass spectrometry. 2,4-D induced the over-expression of at least six C. glutamicum proteins, four of which could be identified by MALDI-TOF-MS. One protein (Cg2521; long-chain acyl-CoA synthetase) was related to the energy metabolism, and two proteins were involved in cell envelope synthesis (Cg2410; glutamine-dependent amidotransferase, and Cg1672; glycosyltransferase). The last induced protein was the ABC type transport system (Cg2695, ATPase component). The newly observed proteins, except for the ABC transport system, were not in general stress-related proteins, but were specifically expressed upon 2,4-D exposure and, therefore, can be used as respective biomarkers. Moreover, since these proteins seem to play a pivotal role in the adaptation of the cell to 2,4-D, they may help to gain deeper insight into the damage mechanisms of 2,4-D induced in the living cell.