Hyperglycemia increases the levels of vascular cellular adhesion molecule-1 and monocyte-chemoattractant-protein-1 in the diabetic endothelial cell
- 作者：Frank Haubner ; Karla Lehle ; Daniela Mü ; nzel ; Christof Schmid ; Dietrich E. Birnbaum ; Jü ; rgen G. Preuner
- 关键词：Endothelial cell ; Diabetes mellitus ; Glucose ; Cell growth ; Adhesion molecules ; Cytokines
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：31 August 2007
- 卷：360
- 期：3
- 页码：560-565
- 文件大小：466 K
摘要
Hyperglycemia is the major cause of diabetic angiopathy. Aim of our study was to evaluate the impact of high glucose on cell growth and function of human “diabetic” endothelial cells (EC). Incubation of non-diabetic EC with glucose moderately inhibited cell growth and increased the expression of ICAM-1 and E-selectin. In the disease-specific EC, glucose treatment resulted also in moderately inhibited cell growth by 5–10%, increased basal expression of VCAM-1 by 10–20%, and an enhanced release of monocyte-chemoattractant-protein-1 (MCP-1) by 40–70%. The expression of ICAM-1 and E-selectin and the release of IL-6 and IL-8 was not affected. The usage of our disease-specific EC model might evaluate the impact of systemic factors of diabetic patients in the progression of endothelial dysfunction, and may be suitable to develop relevant therapeutic regimens.