Effect of essential oils of Zataria multiflora on in vitro rumen fermentation, protozoal population, growth and enzyme activity of anaerobic fungus isolated from Mehraban sheep

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• 作者：R. Talebzadeh^a ; D. Alipour^a ; ^{Alipourd@basu.ac.ir} ; ^{Daryoush.alipour@gmail.com} ; M.J. Saharkhiz^b ; A. Azarfar^c ; M. Malecky^a
• 关键词：A ; asymptotic gas production ; AIVDMD ; apparent in vitro dry matter digestibility ; D144 ; dry matter disappearance after 144  ; h of incubation ; E ; extent of dry matter degradation ; EO ; essential oils ; GP ; gas production ; L ; lag time ; MB ; microbial biomas
• 刊名：Animal Feed Science and Technology
• 出版年：2012
• 期刊代码：7_03778401
• 类别：abs
• 出版时间：30 March, 2012
• 卷：172
• 期：3-4
• 页码：115-124
• 文件大小：548 K

摘要

This study was conducted to investigate the effects of different doses (0, 150, 300, 450 and 600 渭g/ml) of Zataria multiflora essential oils (ZMEO) on in vitro gas production, fermentation parameters and protozoal population using rumen inocula from growing lambs fed a high concentrate diet. Gas production (GP) was recorded at 1-144 h of incubation. After 144 h, the incubation was stopped and inoculants filtered to determine dry matter disappearance (D₁₄₄). In separate runs, the effects of ZMEO on total protozoal count and species distribution were studied. The main constituents of ZMEO were carvacrol (515 g/kg), p-cymene (204 g/kg) and thymol (154 g/kg). Addition of ZMEO reduced asymptotic GP, GP rate, D₁₄₄ and dry matter disappearance (P < 0.001), while GP lag and half-time were increased (P < 0.001). Also GP after 24 h of incubation, ammonia production, microbial biomass and true in vitro degradability were decreased, whereas the partitioning factor increased with addition of ZMEO (P < 0.001). Total VFA was the highest at 150 渭g/ml of ZMEO. Inclusion of ZMEO decreased the number of Entodinium spp., Epidinium caudatum and Epidinium ecaudatum (P < 0.001). At the doses higher than 150 渭g/ml of ZMEO Isotricha spp. as well as Dasytricha spp. were completely disappeared.

Light microscopy showed that the isolated fungi were similar to Neocalimastix sp. Inclusion of ZMEO entirely inhibited growth and filter paper digestion by isolated fungus. The avicelase and 尾-1,4-glucosidase activity in both extracellular and cell associated fractions was decreased by ZMEO (P < 0.01). It is concluded that the ZMEO has the potential to modulate ruminal fermentation and further in vivo research is needed to determine the optimal doses of ZMEO.