Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid
- 作者：Janssen ; M.I. ; van Leeuwen ; M.B.M. ; Scholtmeijer ; K. ; van Kooten ; T.G. ; Dijkhuizen ; L. ; Wö ; sten ; H.A.B.
- 关键词：Biocompatibility ; RGD peptide ; Surface modification ; Self-assembly ; Hydrophobin
- 刊名：Biomaterials
- 出版年：2002
- 期刊代码：7_01429612
- 类别：ms
- 出版时间：December, 2002
- 卷：23
- 期：2
- 页码：4847-4854
- 文件大小：411 K
摘要
Class I Hydrophobins self-assemble at hydrophilic–hydrophobic interfaces into a highly insoluble amphipathic film. Upon self-assembly of these fungal proteins hydrophobic solids turn hydrophilic, while hydrophilic materials can be made hydrophobic. Hydrophobins thus change the nature of a surface. This property makes them interesting candidates to improve physio- and physico-chemical properties of implant surfaces. We here show that growth of fibroblasts on Teflon can be improved by coating the solid with genetically engineered SC3 hydrophobin. Either deleting a stretch of 25 amino acids at the N-terminus of the mature hydrophobin (TrSC3) or fusing the RGD peptide to this end (RGD-SC3) improved growth of fibroblasts on the solid surface. In addition, we have shown that assembled SC3 and TrSC3 are not toxic when added to the medium of a cell culture of fibroblasts in amounts up to 125μgml−1.